Inhibitory effect of pancreatic secretin on gastric secretion.

GREENLEE, HERBERT B., ENRIQUE H. LONGHI, JOSE DELGADILLO GUERRERO, THOMAS S. NELSEN, ABDUL LATIF EL-BEDRI AND LESTER R. DRAGSTEDT. hhibitwy effect of pmwreatic secretiN on gust& secretion. Am. J. Physiol. 190(3) : 396-402. 1957.---In dogs prepared with both a vagus denervated Heidenhain pouch and a total pancreatic fistula, the intravenous injection of pancreatic secretin (Lilly) produced a profuse secretion of pancreatic juice and a simultaneous marked inhibition of gastric secretion. In dogs prepared with an isolated antrum pouch and a Heidenhain pouch the gastric secretion induced by the instillation of food in the antrum pouch was completely inhibited by the intravenous injection of pancreatic secretin. On the other hand, the intravenous injection of pancreatic secretin had little or no effect on the secretion of gastric juice produced by insulin hypoglycemia or by the injection of histamine. It is suggested that pancreatic secretin may represent the mechanism by means of which acid food in the duodenum inhibits gastric secretion. It is probable that this inhibition is caused by prevention of gastrin release from the antrum rather than to a depressant effect on the parietal cells. ABKIN (I) credits Sokolov working in B Pavlov’s laboratory with being the first (1904) to demon&ate that the introduction of gastric juice into the duodenum produced a marked diminution in the secretion of gastric juice from a Pavlov pouch. Subsequently this inhibitory effect of acid in the duodenum on gastric secretion was extensively studied and in general the findings of Sokolov confirmed. Thus Day and Webster (z), found that the introduction of food acidified with gastric juice into the duodenum, surgically disconnected from the stomach, inhibited gastric secretion stimulated either by sham feeding or by the presence of foods in the intestines. Wilhelmj and his associates (3) concluded that inhibition of the gastric and intestinal phases of secretion was marked when acid was introduced into the duodenum whereas the nervous phase of secretion was little affected. GrifUhs (4) reported that the exReceived for publication April 23, 195 7. l This work has been aided by grants from the Otho S. A. Sprague Memorial Institute and from theDivision of Research Grants and Fellowships, National Institutes of Health. perimental introduction of hydrochloric acid solutions directly into the duodenum of man greatly diminished the secretory response of the stomach to an alcohol test meal. For the most part, however, the mechanism of this acid inhibition of gastric secretion appears not to have been extensively studied. The recent finding in our laboratory that the intravenous injection of a preparation of pancreatic secretin obtained from Eli Lilly and Company produced a profuse secretion of pancreatic juice and a simultaneous marked inhibition of gastric secretion in dogs prompted the studies embodied in this report. The observation. appeared interesting in view of the statement by Kosaka and Lim (5) that their preparations of enterogastrone contained no pancreatic secretin. EXPERIMENTAL PROCEDURE Preparations of secretin suitable for intravenous injection in human patients were secured from Eli Lilly and Company and used for the following experiments. The preparation contained IO clinical units of pancreatic secretin per cubic centimeter, I clinical unit 396 by 10.0.33.6 on Jne 1, 2017 http://ajple.physiology.org/ D ow nladed fom PANCREATIC SECRETIN AND GASTRIC SECRETION 397 FIG. I. Effect of an intravenous injection of pancreatic secretin (1.5 u/kg) on the secretion of gastric and pancreatic juice in a dog provided with a total pancreatic fistula and a Heidenhain pouch. A simultaneous stimulation of pancreatic secretion and inhibiof gastric secretion was produced. FIG. 2. Effect of an intravenous injection of pancreatic secretin on gastric secretion in a dog with a Heidenhain pouch. This animal was also provided with an isolated pouch of the antrum which was stimulated continuously during the course of the experiment by the *r instillation of 3% liver solution e-J S every 15 min. Injection of pan.40 f creatic secretin produced a marked 0 temporary depression in gastric secretion . D-292 PANCREATIC SECRETORY RESPONSE TO-SECREflN